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Molecular cloning and characterization of novel phytocystatin gene from turmeric, Curcuma longa


Chan, Seow Neng and Abu Bakar, Norliza and Mahmood, Maziah and Ho, Chai Ling and Shaharuddin, Noor Azmi (2014) Molecular cloning and characterization of novel phytocystatin gene from turmeric, Curcuma longa. BioMed Research International, 2014. art. no. 973790. pp. 1-9. ISSN 2314-6133; ESSN: 2314-6141


Phytocystatin, a type of protease inhibitor (PI), plays major roles in plant defense mechanisms and has been reported to show antipathogenic properties and plant stress tolerance. Recombinant plant PIs are gaining popularity as potential candidates in engineering of crop protection and in synthesizing medicine. It is therefore crucial to identify PI from novel sources like Curcuma longa as it is more effective in combating against pathogens due to its novelty. In this study, a novel cDNA fragment encoding phytocystatin was isolated using degenerate PCR primers, designed from consensus regions of phytocystatin from other plant species. A full-length cDNA of the phytocystatin gene, designated CypCl, was acquired using 5′/3′ rapid amplification of cDNA ends method and it has been deposited in NCBI database (accession number KF545954.1). It has a 687 bp long open reading frame (ORF) which encodes 228 amino acids. BLAST result indicated that CypCl is similar to cystatin protease inhibitor from Cucumis sativus with 74% max identity. Sequence analysis showed that CypCl contains most of the motifs found in a cystatin, including a G residue, LARFAV-, QxVxG sequence, PW dipeptide, and SNSL sequence at C-terminal extension. Phylogenetic studies also showed that CypCl is related to phytocystatin from Elaeis guineensis.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1155/2014/973790
Publisher: Hindawi Publishing Corporation
Keywords: Molecular cloning; Phytocystatin gene; Phytocystatin; Turmeric; Curcuma longa
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 16 Dec 2015 01:58
Last Modified: 16 Dec 2015 01:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2014/973790
URI: http://psasir.upm.edu.my/id/eprint/34575
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