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MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction


Citation

Zainal Abidin, Shahidee and Abbaspourbabaei, Maryam and Ntimi, Carolindah Makena and Siew, Wei Hong and Cheah , Pike See and Rosli, Rozita and Nordin, Norshariza and Ling, King Hwa (2014) MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction. Malaysian Journal of Medical Sciences, 21 (spec.). pp. 27-33. ISSN 1394-195X; ESSN: 2180-4303

Abstract

Background: MicroRNAs (miRNAs) have a crucial role in gene expression regulation and protein synthesis, especially in the central nervous system. In developing mouse embryos a novel miRNA, miR-3099, is highly expressed, particularly in the central nervous system. This study aims to determine the expression of miR-3099 during cellular differentiation of 46C mouse embryonic stem cells after neural induction with N2/B27 medium. Methods: 46C mouse embryonic stem cells were subjected to neural induction with N2/B27 medium. At 0, 3, 7, 11, 17, and 22 days after neural induction, the cells were screened for various pluripotent, progenitor, and differentiating/differentiated cells markers by immunocytochemistry and reverse-transcriptase polymerase chain reaction (RT-PCR). Stem-loop pulse RT-PCR was performed to determine the expression of miR-3099 at all selected time points after neural induction. Results: Our findings showed that after induction, mouse embryonic stem cells differentiated into heterogeneous pools of cells containing neurons, astrocytes, and oligodendrocytes. Mouse embryonic stem cells and neural progenitor/precursor cells were also present in culture up to day 22 as indicated by RT-PCR analysis. Elucidation of miR-3099 expression during in vitro neural induction revealed that this miRNA was expressed throughout the differentiation process of 46C mouse embryonic stem cells. miR-3099 was expressed at higher levels on day 11, 17, and 22 as compared to day 0, 3 and 7 after neural induction. Conclusion: The level of miR-3099 expression was higher in differentiated mouse embryonic stem cells after neural induction. This finding suggested that miR-3099 might play a role in regulating neural stem cell differentiation. However, further characterisation of miR-3099 in a better characterised or optimised differentiated neural stem cell culture would provide increased understanding of the cellular function and molecular targets of miR-3099, especially in neuron development.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Penerbit Universiti Sains Malaysia
Notes: Special issue - Neuroscience
Keywords: MicroRNA; MiR-3099; Neural induction; 46C mouse embryonic stem cells; Neuron
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 15 Dec 2015 08:36
Last Modified: 15 Dec 2015 08:50
URI: http://psasir.upm.edu.my/id/eprint/34528
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