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Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays


Citation

Khoo, Hock Eng and Azlan, Azrina and Ismail, Amin and Abas, Faridah and Hamid, Muhajir (2014) Inhibition of oxidative stress and lipid peroxidation by anthocyanins from defatted Canarium odontophyllum pericarp and peel using in vitro bioassays. PLOS ONE, 9 (1). art. no. e81447. pp. 1-9. ISSN 1932-6203

Abstract / Synopsis

Canarium odontophyllum, also known as CO, is a highly nutritious fruit. Defatted parts of CO fruit are potent sources of nutraceutical. This study aimed to determine oxidative stress and lipid peroxidation effects of defatted CO pericarp and peel extracts using in vitro bioassays. Cell cytotoxic effect of the CO pericarp and peel extracts were also evaluated using HUVEC and Chang liver cell lines. The crude extracts of defatted CO peel and pericarp showed cytoprotective effects in t-BHP and 40% methanol-induced cell death. The crude extracts also showed no toxic effect to Chang liver cell line. Using CD36 ELISA, NAD+ and LDL inhibition assays, inhibition of oxidative stress were found higher in the crude extract of defatted CO peel compared to the pericarp extract. Hemoglobin and LDL oxidation assays revealed both crude extracts had significantly reduced lipid peroxidation as compared to control. TBARS values among defatted CO pericarp, peel, and cyanidin-3-glucoside showed no significant differences for hemoglobin and LDL oxidation assays. The protective effects of defatted CO parts, especially its peel is related to the presence of high anthocyanin that potentially offers as a pharmaceutical ingredient for cardioprotection.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Halal Products Research Institute
DOI Number: https://doi.org/10.1371/journal.pone.0081447
Publisher: Public Library of Science
Keywords: Oxidative stress; Lipid peroxidation; Anthocyanins; Canarium odontophyllum pericarp; Vitro bioassays
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 18 Jan 2016 13:39
Last Modified: 18 Jan 2016 13:39
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1371/journal.pone.0081447
URI: http://psasir.upm.edu.my/id/eprint/34212
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