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Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross”


Citation

Ravanfar, Seyed Ali and Salim, Shahida and Abdul Aziz, Maheran and Abdullah, Siti Nor Akmar and Abdul Rashid, Azmi (2014) Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross”. Plant Biotechnology, 31 (3). pp. 275-280. ISSN 1342-4580; ESSN: 1347-6114

Abstract

In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6- y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of Brassica oleracea ssp. capitata “KY Cross” were investigated. For hypocotyl explants, medium containing 2.27 μM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 μM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage “KY Cross”.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.5511/plantbiotechnology.14.0514a
Publisher: Japanese Society for Plant Cell and Molecular Biology
Keywords: Adeninetype cytokinin; Brassica oleracea subsp. capitata “KY Cross”; Hypocotyl; Phenyl-urea cytokinin; Shoot organogenesis
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 15 Sep 2016 03:05
Last Modified: 15 Sep 2016 03:05
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5511/plantbiotechnology.14.0514a
URI: http://psasir.upm.edu.my/id/eprint/34197
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