UPM Institutional Repository

Binding of copper (II) ion to cytochrome c


Augustin, Mary Ann and Yandell, John K. (1981) Binding of copper (II) ion to cytochrome c. Australian Journal of Chemistry, 34 (1). pp. 91-97. ISSN 0004-9425


Binding of aquacopper(11) ion to horse and tuna ferricytochrome c has been investigated over the pH range 2-9. Between pH 4 and 7 the principal binding site for horse cytochrome has an intrinsic association constant of 1.8 × 104 1. mol-1 and pKa of 6.5±0.6 at 25ºC and ionic strength of 0.1. Binding to histidine 33 is consistent with this information, as well as with e.s.r., n.m.r. and visible absorption spectral data. Other sites also bind copper but with association constants at least a factor of ten smaller. At high pH (> 8) further binding sites predominate.

Download File

[img] PDF
34013 binding of copper.pdf
Restricted to Repository staff only

Download (2MB)

Additional Metadata

Item Type: Article
Divisions: Universiti Pertanian Malaysia
Faculty of Food Science and Technology
Publisher: CSIRO Publishing
Keywords: Copper; Cytochrome c
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 15 Apr 2015 04:29
Last Modified: 15 Apr 2015 04:29
URI: http://psasir.upm.edu.my/id/eprint/34013
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item