Some kinetic studies on immobilized uricase

Citation

Salleh, Abu Bakar and Ledingham, W. M. (1981) Some kinetic studies on immobilized uricase. International Journal of Biochemistry, 13 (10). pp. 1113-1118. ISSN 0020-711X

Abstract

1. Uricase was immobilized onto glutaraldehyde-activated nylon tube. 2. The activity of the immobilized uricase tube was assayed by a plug-flow method adopting the integrated form of Michaelis-Menten equation. 3. Up to about 60% activity of the soluble enzyme is retained when the enzyme is derivatized. 4. Effects of changing certain parameters in the assay system were also shown. 5. The immobilized uricase has an optium pH of about 9.0, and is quite stable at 37°C when stored in borate buffer pH 9.0 in the presence of NaCl and Triton × 100.

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Additional Metadata

Item Type:	Article
Divisions:	Universiti Pertanian Malaysia
DOI Number:	https://doi.org/10.1016/0020-711X(81)90175-0
Publisher:	Elsevier
Keywords:	Uricase
Depositing User:	Mohd Hafiz Che Mahasan
Date Deposited:	14 Apr 2015 04:51
Last Modified:	14 Apr 2015 04:51
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/0020-711X(81)90175-0
URI:	http://psasir.upm.edu.my/id/eprint/33896
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