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Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus


Citation

Mohamed Shariff Mohamed Din (2013) Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus. PI2013702063.

Abstract / Synopsis

The present invention provides a loop-mediated isothermal amplification (LAMP) method for the detection of infectious spleen and kidney necrosis virus (ISKNV) infection by amplifying a portion of ISKNV major capsid protein (MCP) gene using isothermal amplification and detecting the amplified product by acridine orange. Newly designed primers for use with this specific method are provided as well.The LAMP assay of the invention is specific towards ISKNV and there was no cross-amplification with other pathogens including the RSIV.


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Additional Metadata

Item Type: Patent
Application Number: PI2013702063
Filing Date: 2013-10-29
Divisions: Institute of Bioscience
Keywords: Loop-mediated isothermal amplification (LAMP); Infectious spleen; Kidney necrosis virus (ISKNV)
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 31 Mar 2015 09:01
Last Modified: 31 Mar 2015 09:01
URI: http://psasir.upm.edu.my/id/eprint/33752
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