Citation
Abdul Razak, Che Nyonya and Tang, S. W. and Basri, Mahiran and Salleh, Abu Bakar
(1997)
Preliminary Study on the Production of Extracellular Protease
from a Newly Isolated Bacillus sp. (No.1) and the Physical
Factors Affecting Its Production.
Pertanika Journal of Science & Technology, 5 (2).
pp. 169-177.
ISSN 0128-7680
Abstract
A thermophilic bacterium isolated from a dumping ground in Sri Petaling, Kuala Lumpur, identified as Bacillus sp. (No.1), was able to grow at 600C and showed proteolytic activity on skim milk agar. The protease production by this
bacterium and some physical factors affecting its production were investigated. Significant protease activity was detected in the basal medium after incubation
for 16 h at 50°C with shaking at 200 rpm and reached its peak after 30 h. The protease production coincided with the late exponential or early stationary
phase of bacterial growth and corresponded with the sporulation of this bacterium. The optimum pH, agitation rate and temperature for protease
production by Bacillus sp. (No.1) were pH 8, 150 rpm and 50°C, respectively.
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Additional Metadata
Item Type: |
Article
|
Publisher: |
Universiti Putra Malaysia Press |
Keywords: |
extracellular protease, production, physical factors, Bacillus sp.
|
Depositing User: |
Nur Izzati Mohd Zaki
|
Date Deposited: |
25 Nov 2009 07:34 |
Last Modified: |
27 May 2013 07:07 |
URI: |
http://psasir.upm.edu.my/id/eprint/3368 |
Statistic Details: |
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