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Capacitation status of frozen thawed Bali bull (Bos Javanicus) spermatozoa with different heparin concentrations


Sarsaifi, Kazhal and Harron, Abd. Wahid and Yusoff, Rosnina and Hani, Homayoun and Yimer, Nurhusien and Naing, Soh Win and Vejayan, Jaya and Osman, Abas Mazni (2012) Capacitation status of frozen thawed Bali bull (Bos Javanicus) spermatozoa with different heparin concentrations. In: International Conference On One Health & 24th VAM Congress 2012 , 21-23 Sep. 2012, Marriott Putrajaya, Malaysia. (pp. 67-68). (Unpublished)


The present study was conducted to assess the capacitation status of frozen-thawed Bali spermatozoa in different heparin concentration with a short incubation time. Frozen thawed spermatozoa from Bali bulls were treated with either 25, 50 or 100 ug/ml heparin for 30 min. Capacitation was evaluated by acrosome reaction of spermatozoa and heterologous in vitro fertilization test] fertilization percentage (FP) and fertilization index (FI)]. Acrosome reaction was induced in heparin treated spermatozoa with calcium ionophore A23187 and staining was carried out with naphthol yellow S- erythrosine B to evaluate the response as compared with control (without heparin). Significantly higher percentage of acrosome reaction (AR) spermatozoa was observed after heparin treatment (43.8 - 60.2%) as compared with control (11.6%; p < 0.01). Also, a significantly higher in vitro fertilization rate was recorded in spermatozoa capacitated by 50 and 100 ug/ml heparin (68.4 and 73.6% fertilization percentage, respectively) as compared with control and 25 ug/ml heparin (13.5 and 41% cleavage rate; p < 0.01). It is concluded that Bali spermatozoa capacitated with 50-100 ug/ ml heparin had significantly higher ability to improve capacitation status and acrosome reaction.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Keywords: Heparin; Heterologous in vitro fertilization; Bali spermatozoa.
Depositing User: Azian Edawati Zakaria
Date Deposited: 10 Mar 2015 07:03
Last Modified: 12 Mar 2015 09:08
URI: http://psasir.upm.edu.my/id/eprint/33605
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