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Purified T1 lipase, a novel construct of T1 lipase and methods thereoF


Raja Noor Zaliha Raja Abd. Rahman (2011) Purified T1 lipase, a novel construct of T1 lipase and methods thereoF. PI2011700160.


The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). This is to enable to diferentiate the isoelectric point (pI) value of GST from the pI value of matured T1 lipase. Also, by obtaining a new construct, new T1 lipase from the above methods, the new construct and T1 lipase was purified using two chromatography steps affinity and ion exchange chromatography. Thus, as mentioned above, the said method provides an advantage to limit or eliminate the second affinity chromatography step.

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Additional Metadata

Item Type: Patent
Application Number: PI2011700160
Filing Date: 2011-11-09
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Keywords: Lipase; T1 lipase; Chromatography
Depositing User: Suzila Mohamad Kasim
Date Deposited: 23 Dec 2014 08:17
Last Modified: 27 Sep 2016 01:30
URI: http://psasir.upm.edu.my/id/eprint/33123
Statistic Details: View Download Statistic

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