Simple Search:

A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof


Citation

Annie Christianus (2011) A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof. PI2011005989.

Abstract / Synopsis

The present invention relates to a method and a kit for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile. The method comprising: preparing a volume of bile sample from a fish; adding a volume of enzyme combination and a volume of distilled water to the volume of bile in order to obtain a mixture; incubating the mixture; adding a volume of acetone to the mixture; centrifuging the mixture and obtaining a supernatant and a pellet; and recovering the biliary FACs in the supernatant using high performance liquid chromatography with fluorescent detection (HPLC/FL). To increase accuracy of the method, pellets resulted from the centrifugation were resuspended in distilled water and the above-mentioned procedure was repeated but using different ratios of distilled water, enzyme combination, and acetone. The highly accurate result is required for accurate estimation of PAHs concentrations in aquatic environments. In addition, early estimation of low concentrations of PAHs in fish products through fish bile ensures the extra safe consumption of fish products by human and farm animals.


Download File

Full text not available from this repository.

Additional Metadata

Item Type: Patent
Application Number: PI2011005989
Filing Date: 2011-12-09
Divisions: Faculty of Agriculture
Keywords: Pollution; Aquatic; Fluorescent aromatic compounds; Fish bile; High performance liquid chromatography; Fluorescent detection
Depositing User: Suzila Mohamad Kasim
Date Deposited: 23 Dec 2014 15:54
Last Modified: 23 Dec 2014 15:54
URI: http://psasir.upm.edu.my/id/eprint/33119
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item