UPM Institutional Repository

Thermostable proteases


Ibrahim, Noor Azlina and Leow, Adam Thean Chor and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2006) Thermostable proteases. In: New Lipases and Proteases. Nova Science Publishers, New York, pp. 77-93. ISBN 9781600210686


New proteases are constantly isolated. Proteases, mainly the alkaline proteases are widely used in the detergent formulation. Thermostable alkaline proteases are of great interest in the detergent industry because these enzymes are stable and able to retain its activities over broad range of pH and temperatures. Most thermostable alkaline proteases reported are from Bacillus spp. In this chapter, we introduce a new thermostable alkaline protease produced from Bacillus stearothermophilus strain F1. The protease production requirements by strain F1, the properties and characterization of this enzyme are discussed. The protease F1 gene was successfully cloned and expressed into E. coli XL1-Blue. The bacteriocin release protein(BRP)system was utilized; to release the recombinant F1 protease into the culture medium. The purified native and recombinant F1 protease showed a pH optimum of 9.0 and thermostabilities ranging from a half-life of 25 min at 90◦C to a half-life of 4 h at 85◦C. The optimumtemperatures were 85◦C and 80◦C respectively.

Download File

PDF (Abstract)
Abstract-Thermostable proteases.pdf

Download (184kB) | Preview

Additional Metadata

Item Type: Book Section
Call Number: QP609 L5N532
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Nova Science Publishers
Keywords: Thermostable proteases; Proteases
Depositing User: Nadiana Adlia Rusli
Date Deposited: 20 Aug 2015 06:21
Last Modified: 26 Sep 2016 04:07
URI: http://psasir.upm.edu.my/id/eprint/32974
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item