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Quantification and antibiotic susceptibility of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium in raw vegetables (ulam)


Citation

Mohd Shahril, Najwa and Rukayadi, Yaya and Anyi, Ubong and Loo, Yuet Ying and Chang, Wei San and Lye, Ying Ling and Thung, Tze Young and Anuar, Aimi Syazana and Tan, Malcolm Turk Hsern and Goh, Sur Guat and Kuan, Chee Hao and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2015) Quantification and antibiotic susceptibility of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium in raw vegetables (ulam). International Food Research Journal, 22 (5). pp. 1761-1769. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Salmonella has been reported to be presence both in raw and processed foods worldwide. In this study, the prevalence, quantification and antibiotic susceptibility of Salmonella isolated from raw vegetables or locally known as ulam such as asiatic pennywort (Centella asiatica (L) Urb), water dropwort (Oenanthe javanica (Blume) DC), long bean (Vigna sinensis EndL), and winged bean (Psophocarpus tetragonolobus (L) DC) obtained from retail markets in Selangor, Malaysia were carried out. From 96 samples tested, the overall prevalence of Salmonella spp. was 97.9%, Salmonella Enteritidis was 54.2% and Salmonella Typhimurium was 82.3% respectively. Samples were contaminated with Salmonella ranging from <3 to 2400 MPN/g. Salmonella Enteritidis and Salmonella Typhimurium isolates obtained from the raw vegetables (ulam) were found to exhibit high resistance against ampicillin (100%), erythromycin (100%), amoxicillin/clavunic acid (81.3%), cephalothin (75%), streptomycin (50%) and ciprofloxacin (50%). All Salmonella isolates showed multi drug resistant (MDR) profile with each isolate being resistant to 3 or more antibiotics. The multiple antibiotic resistance (MAR) index of Salmonella isolates ranged from 0.27 to 0.55 for Salmonella Enteritidis and 0.27 to 0.82 for Salmonella Typhimurium. The presence of Salmonella on raw vegetables (ulam) and high antibiotic resistance isolates indicated that raw vegetables could be contaminated and thus imposes possible health risk to local consumers.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Antibiotic; Prevalence; Salmonella enteritidis; Salmonella spp; Salmonella typhimurium
Depositing User: Nabilah Mustapa
Date Deposited: 27 Jan 2016 02:21
Last Modified: 27 Jan 2016 02:21
URI: http://psasir.upm.edu.my/id/eprint/32859
Statistic Details: View Download Statistic

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