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Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions


Citation

Rashid Ibrahim (2010) Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions. PI2010700063.

Abstract / Synopsis

The present invention provides a method of in vitro cultivation of mesenchymal stem cells into osteoblasts, in which total bone marrow taken from a mammal is cultivated in a complete medium containing an increased amount of fetal bovine serum under standard conditions, subsequently the non-adherent cells are removed and the adherent cells are further expanded in a complete medium containing an increased amount of fetal bovine serum and after the expansion they are cultivated in an osteogenic inducing medium containing an increased amount of fetal bovine serum under standard conditions. The method is suitable also for bone marrow taken from a health-compromised mammal.


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Additional Metadata

Item Type: Patent
Application Number: PI2010700063
Filing Date: 2010-09-08
Divisions: Faculty of Veterinary Medicine
Keywords: Vitro isolation; Mesenchymal stem cells
Depositing User: Junaini Mohd Yusof
Date Deposited: 03 Oct 2014 02:51
Last Modified: 21 Nov 2016 04:15
URI: http://psasir.upm.edu.my/id/eprint/32141
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