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A method for detecting candida species in a specimen by polymerase chain reaction.


Citation

Chong Pei Pei (2011) A method for detecting candida species in a specimen by polymerase chain reaction. PI2011000631.

Abstract / Synopsis

The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detecting the amplified nucleic acid for each Candida species; characterized in that, in a single reaction, the DNA primers consist of: SEQ ID NO. 1 and SEQ ID NO. 2 or their complements for amplifying nucleic acid from Candida albicans; SEQ ID NO.3 and SEQ ID NO. 4 or their complements for amplifying nucleic acid from Candida glabrata; SEQ ID NO. 5 and SEQ ID NO. 6 or their complements for amplifying nucleic acid from Candida parapsilosis; SEQ ID NO. 7 and SEQ ID NO. 8 or their complements for amplifying nucleic acid from Candida tropicalis.


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Additional Metadata

Item Type: Patent
Application Number: PI2011000631
Filing Date: 2011-02-11
Divisions: Faculty of Medicine and Health Science
Keywords: Candida species; Polymerase chain reaction; DNA primers.
Depositing User: Suzila Mohamad Kasim
Date Deposited: 15 Oct 2014 16:18
Last Modified: 15 Oct 2014 16:18
URI: http://psasir.upm.edu.my/id/eprint/32082
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