UPM Institutional Repository

Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer


Mohd Yusof, Aziah and Ab Shukor, Nor Aini and Awang, Kamis and Abdul Kadir, Mihdzar (2013) Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer. Pertanika Journal of Tropical Agricultural Science, 36 (S). pp. 67-78. ISSN 1511-3701; ESSN: 2231-8542


The greatest drawback in large scale micropropagation of tropical woody forest species is high contamination of cultures. In developing a sterilization protocol for micropropagation of Shorea parvifolia Dyer utilizing nodal segments excised from nursery-grown seedlings, it was found that washing 20% (v/v) with Clorox solution for 18 minutes was the best. After six weeks of culture in WPM media supplemented with 10-5 M BAP (apart from fungal and bacterial contamination), the nodal segments developed hair-like structures which were amenable to subculture. Upon subculture, green leafy structures developed from the mass of hairy structure after six weeks. These later developed into ferns which are normally found as epiphytes on older forest trees, known as Asplenium nidus.

Download File

6. Incidence of Fern Contamination in Nodal Segment Cultures.pdf

Download (767kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Forestry
Faculty of Agriculture
Institute of Tropical Forestry and Forest Products
Publisher: Universiti Putra Malaysia Press
Keywords: Shorea parvifolia; Sterilization; Contamination; Asplenium nidus
Depositing User: Muizzudin Kaspol
Date Deposited: 25 Nov 2015 03:41
Last Modified: 25 Nov 2015 03:41
URI: http://psasir.upm.edu.my/id/eprint/31354
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item