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Molecular and morphological detection of Plasmodium species in wild macaques in Selangor, Malaysia


James Abie, Marsyia and Sharma, Reuben Sunil Kumar and Ho, Gim Chong and Zainuddin, Zainal Zahari (2010) Molecular and morphological detection of Plasmodium species in wild macaques in Selangor, Malaysia. In: 5th Seminar in Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. .


Malaria is a serious global health problem, and rapid, accurate diagnosis is required to control the disease. A number of methods have been developed in recent years for diagnosing this disease, including polymerase chain reaction (PCR)-based technique that detect specific nucleic acid sequences, and microscopic examination of thin blood films that remains the most widely and commonly used method. In this study, blood samples were collected from 125 wild macaques consisting of 18 Macaca nemestrina and 107 M. fasicularis from various areas of Selangor. Giemsa-stained thin blood films (TBF) were prepared, and PCR using Plasmodium genus-specific primers for initial amplification and nested species-specific primers for Plasmodium knowlesi was conducted on all the blood samples. The prevalence of Plasmodium by TBF was 1.9% in M. fasicularis and 27.8% in M. nemestrina. The molecular prevalence of Plasmodium was 64.5% in M. fasicularis and 100% in M. nemestrina. When P. knowlesi-specific PCR was carried out, the prevalence in M. nemestrina was 5.6%, whereas in M. fasicularis it was 23.3%. These results indicate that the local wild macaques harbor a high rate of infection of Plasmodium. In addition, the prevalence of P. knowlesi, the zoonotic malaria parasite is higher than previously assumed. This warrants further investigation as these macaques may be potential reservoirs of human malaria in Malaysia.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Veterinary Medicine
Keywords: Polymerase chain reaction (PCR); Thin blood films; Plasmodium knowlesi; Macaca nemestrina; Macaca fasicularis
Depositing User: Azizan Arshad
Date Deposited: 16 Jun 2014 03:59
Last Modified: 08 Jan 2015 10:17
URI: http://psasir.upm.edu.my/id/eprint/30955
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