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Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology


Citation

Chen, Wei Li and Liang, Juan Boo and Jahromi, Mohammad Faseleh and Ho, Yin Wan and Abdullah, Norhani (2013) Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology. BioResources, 8 (3). pp. 3844-3857. ISSN 1930-2126

Abstract

Response surface methodology (RSM) was used to optimize the co-production of a mixture of crude cellulosic and hemicellulosic enzymes (endoglucanase, xylanase, and mannanase) by Aspergillus terreus K1 in solid-state fermentation (SSF) using palm kernel expeller (PKE) as the sole carbon source. These enzymes have gained renewed interest due to their efficacy to improve the digestibility of PKE for use in diets of mono-gastric animals (poultry, pigs, and fish). The results showed that temperature, moisture, inoculum concentration, and initial pH had significant (P< 0.05) effects on the enzymes production. Using PKE as a solid substrate, maximum endoglucanase, mannanase, and xylanase (17.37, 41.24, and 265.57 U/g DM, respectively) were obtained at 30.5 °C, 62.7% moisture, 6% inoculum, and pH 5.8. The enzyme activities recorded were close to the predicted values (19.97, 44.12, and 262.01 U/g DM, respectively).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Institute of Tropical Agriculture
Publisher: North Carolina State University
Keywords: Solid-state fermentation; Aspergillus terreus; Palm kernel expeller; Response surface methodology
Depositing User: Umikalthom Abdullah
Date Deposited: 01 Apr 2015 03:21
Last Modified: 07 Feb 2020 01:07
URI: http://psasir.upm.edu.my/id/eprint/30724
Statistic Details: View Download Statistic

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