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Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia


Citation

Loo, Yuet Ying and Soopna, Puspanadan and Goh, Sur Guat and Kuan, Chee Hao and Chang, Wei San and Lye, Ying Ling and Tang, John Yew Huat and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2013) Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia. International Food Research Journal, 20 (6). 3313 - 3317. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Foodborne diseases are mainly caused by bacterial contamination which can lead to severe diarrhea. This study aimed to detect the presence of Shiga toxin-Producing Escherichia coli O157, Escherichia coli non-O157 and virulence gene in raw vegetables. The samples were purchased from wet market and hypermarket in Selangor. The detections were carried out by using the combination methods of Most Probable Number-Polymerase Chain Reaction (MPN- PCR). A total of 37(18.5%) samples were found to be contaminated by STEC. Out of these 37 isolates, four (10.8%) of the isolates were E. coli O157 while 33(89.2%) were E. coli non- O157. However, there was no E. coli O157:H7 detected in all the samples. The occurrence of Shiga toxin-Producing E. coli in edible raw vegetables samples suggests the importance of this pathogen in vegetables. Therefore, more studies are required to remove this pathogen from vegetables.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Characterization; Escherichia coli; Shiga-toxin; Raw vegetables; MPN-PCR
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 28 Dec 2014 06:29
Last Modified: 02 Jun 2015 03:45
URI: http://psasir.upm.edu.my/id/eprint/30525
Statistic Details: View Download Statistic

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