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Effects of extraction solvent system, time and temperature on total phenolic content of henna (Lawsonia inermis) stems


Tan, Mun Chieng and Tan, Chin Ping and Ho, C. W. (2013) Effects of extraction solvent system, time and temperature on total phenolic content of henna (Lawsonia inermis) stems. International Food Research Journal, 20 (6). pp. 3117-3123. ISSN 1985-4668; ESSN: 2231-7546


Henna plant ( Lawsonia inermis ) is an Indian medicinal plant used in traditional medicine for the treatment of various diseases, besides its popularity as a natural dye to colour hand and hair. Research in the recent past has accumulated enormous evidence revealing henna plant to be an excellent source of antioxidants such as total phenolics. In this study, the extraction of total phenolics from henna stems was evaluated using the Folin-Ciocalteu assay. A set of single factor experiments was carried out for identifying the optimum condition of each independent variable affecting total phenolic content (TPC) extraction efficiency of henna stems, namely the solvent type, solvent concentration (v/v, %), extraction time (min) and extraction temperature ( o C). Generally, high extraction yield was obtained using aqueous acetone (about 40%) as solvent and the extraction yield could further be increased using a prolonged time of 270 min and a higher incubation temperature of 55°C. Under these optimized conditions, the experimental maximum yield of TPC of 5554.15 ± 73.04 mg GAE/100 g DW was obtained.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Henna (Lawsonia inermis) stems; Total phenolic content (TPC); Solvent extraction; Folin-ciocalteu assay
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 28 Dec 2014 06:15
Last Modified: 03 Jun 2015 06:11
URI: http://psasir.upm.edu.my/id/eprint/30521
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