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Optimal binary solvent extraction system for phenolic antioxidants from mengkudu (Morinda citrifolia) fruit.


Citation

Thoo, Yin Yin and Ho, Swee Kheng and Abas, Faridah and Lai, Oi Ming and Ho, Chun Wai and Tan, Chin Ping (2013) Optimal binary solvent extraction system for phenolic antioxidants from mengkudu (Morinda citrifolia) fruit. Molecules, 18 (6). pp. 7004-7022. ISSN 1420-3049

Abstract

Antioxidants have been widely used in the food industry to enhance product quality by preventing oxidation of susceptible substances. This work was carried out to maximise the recovery of total phenolic content (TPC), total flavonoid content (TFC), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical-scavenging capacity and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity from Morinda citrifolia fruit via modification of the ethanol concentration, extraction time and extraction temperature at minimal processing cost. The optimised conditions yielded values of 881.57 ± 17.74 mg GAE/100 g DW for TPC, 552.53 ± 34.16 mg CE/100 g DW for TFC, 799.20 ± 2.97 µmol TEAC/100 g DW for ABTS and 2,317.01 ± 18.13 µmol TEAC/100 g DW for DPPH were 75% ethanol, 40 min of time and 57 °C. The four responses did not differ significantly (p > 0.05) from predicted values, indicating that models obtained are suitable to the optimisation of extraction conditions for phenolics from M. citrifolia. The relative amounts of flavonoids were 0.784 ± 0.01 mg quercetin/g of extract and 1.021 ± 0.04 mg rutin/g of extract. On the basis of the results obtained, M. citrifolia extract can be used as a valuable bioactive source of natural antioxidants.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3390/molecules18067004
Publisher: MDPI
Keywords: Mengkudu (Morinda citrifolia); Total phenolic content (TPC); Total flavonoid content (TFC); 2,2`-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical-scavenging capacity.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 22 Aug 2014 03:19
Last Modified: 12 Jan 2016 00:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules18067004
URI: http://psasir.upm.edu.my/id/eprint/30460
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