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Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves


Zareen, Seema and Ismail, Intan Safinar and Shaari, Khozirah and Yusof, Khairul Nazri and Akhtar, Muhammad Nadeem (2013) Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves. International Journal of Food Properties, 16 (8). pp. 1830-1838. ISSN 1094-2912; ESSN: 1532-2386


A high performance liquid chromatography procedure for the quantitative determination of three marker benzene derivatives, 2,4,6-trihydroxy-3-prenyl acetophenone (tHPA) (1), 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) (2), and p-O-geranyl coumaric acid (GCA) (3), in the Melicope ptelefolia ethanolic leaf extracts, a medicinal herb obtained from a few locations of the Peninsula Malaysia, was described. The quantitative analysis was performed using high performance liquid chromatography-photodiode array detection on Xterra octadecylsiyl silica (ODS; 3.0 × 150 mm, 3.5 μm) column kept at 32°C, using gradient elution with acetonitrile and water containing 0.1% formic acid at a flow-rate of 1 ml/min with UV detection wavelength at 280 nm. All calibration curves showed good linearity (R2 of 0.999 to 1.0000) within the concentrations range of 2.5 × 10-3 to 0.1 mg/mL. The method was shown to be simple, sensitive, and reliable for qualitative and quantitative analysis of the marker compounds in M. ptelefolia leaf preparations.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.1080/10942912.2011.609952
Publisher: Taylor & Francis
Keywords: Quantitative analysis; HPLC-DAD; Melicope ptelefolia; p-O-geranyl coumaric acid (GCA).
Depositing User: Umikalthom Abdullah
Date Deposited: 10 Sep 2014 08:05
Last Modified: 17 Nov 2015 03:43
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/10942912.2011.609952
URI: http://psasir.upm.edu.my/id/eprint/30260
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