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DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode


Citation

Dutse, Sabo Wada and Yusof, Nor Azah and Ahmad , Haslina and Hussein, Mohd Zobir and Zainal, Zulkarnain and Hushiarian, Roozbeh (2013) DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode. International Journal of Electrochemical Science, 8 (9). pp. 11048-11057. ISSN 1452-3981

Abstract

An electrochemical DNA biosensor has been developed for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium [Ru(phen)2,(qtpy)]2+ complex as hybridization indicator. The sensor incorporated the use of a gold electrode (AuE), modified with a conducting nanocomposite of poly(3,4-ethylene-dioxythiophen) -poly(styrenesulfonate) (PEDOT-PSS) and silver nanoparticles (AgNPs). A specific sequence of a ganoderma boninense DNA probe has been immobilized on the modified electrode and the hybridization event was monitored via intercalation of the ruthenium complex to the hybridized DNA. Effect of hybridization temperature and time was evaluated and found to be optimal at 45 °C in 25 minutes for the hybridization. Detection of target DNA ranged from 1.0 x 10-15 M to 1.0 × 10-9 M was performed, and a correlation relationship of 0.9756 and detection limit of 5 × 10-16 M were obtained. The newly synthesized ruthenium complex was able to be used is a novel redox marker and can be adopted for routine detection of DNA.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Institute of Advanced Technology
Institute of Bioscience
Publisher: Electrochemical Science Group
Keywords: Biosensor; Ganoderma boninense; DNA; PEDOT-PSS; Ruthenium complex.
Depositing User: Umikalthom Abdullah
Date Deposited: 25 Aug 2014 04:39
Last Modified: 07 Oct 2015 06:54
URI: http://psasir.upm.edu.my/id/eprint/30183
Statistic Details: View Download Statistic

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