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Characterization of wound responsive genes in Aquilaria malaccensis.


Wong, Mun Theng and Siah, Chai Har and Qamaruz Zaman, Faridah and Mohamed, Rozi (2013) Characterization of wound responsive genes in Aquilaria malaccensis. Journal of Plant Biochemistry and Biotechnology, 22 (2). pp. 168-175. ISSN 0971-7811; ESSN: 0974-1275


We report on the isolation and characterization of several genes responsive to wounding in the tropical endangered tree Aquilaria malaccensis. Wounding triggers the formation of a fragrant substance inside the tree stem. Deduced amino acid of the cloned sequences exhibited sequence similarities to their respective homologs: transcription factors of the WRKY gene family (AmWRKY) and β-1,3-glucanase (AmGLU). A homolog to phenylalanine ammonia-lyase (AmPAL) from previous work was also included. All cDNA sequences were of partial lengths. We studied their expression profiles in a wounding-stress experiment. Mechanical wounding induces AmWRKY in an early response to wounding (3 h), and elevates AmPAL and AmGLU expressions after 16 h. It is possible that AmWRKY mediates early wounding response while AmPAL mediates response to fungal infection by co-inducing AmGLU. Their homologs in other plants are known to inhibit fungal growth. Our data provide the first insight into the mechanisms of wounding responses in Aquilaria.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Faculty of Forestry
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s13562-012-0144-z
Publisher: Springer Verlag
Keywords: Agarwood; Aquilaria malaccensis; Genes; Wounding.
Depositing User: Umikalthom Abdullah
Date Deposited: 30 May 2014 00:26
Last Modified: 09 Oct 2015 01:02
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s13562-012-0144-z
URI: http://psasir.upm.edu.my/id/eprint/29989
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