UPM Institutional Repository

Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia


Elshafie, E. I. and Abdullah Sani, Rehana and Hassan, Latiffah and Kumar Sharma, Reuben Sunil and Fateh Mohamed, Bashir Ahmad and Abubakar, I. A. (2013) Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia. Tropical Biomedicine, 30 (3). pp. 444-450. ISSN 0127-5720


Apart from occasional reports of clinical disease affecting horses, there is no information about Trypanosoma evansi in horses in Peninsula Malaysia. Thus, a cross-sectional study was conducted in eight states in Peninsula Malaysia to determine the active presence of T. evansi in horses. A total of 527 blood samples were obtained and examined by haematocrit centrifugation technique (HCT), Giemsa-stained thin blood smear (GSS), morphometric measurements, polymerase chain reaction (PCR) and cloning of PCR products. The results showed an overall parasitological prevalence of 0.57% (3/527, CI: 1.6-0.19%) with both HCT and GSS. Morphometric study revealed the mean total length of the trypanosomes including the free flagellum was 27.94 ± 2.63 µm. PCR successfully amplified a trypanosome specific 257 bp in 1.14% of samples (6/527, CI: 2.4-0.52%) and was confirmed by nucleotide sequences. The mean packed cell volume (PCV) for the positive cases detected by HCT was lower (23% ± 7.00) compared to the positive cases detected by PCR alone in the state of Terengganu (35% ± 4.73). In conclusion, this study showed T. evansi infection occurred in low frequency in horses in Peninsula Malaysia, and anaemia coincided with parasitaemic animals. PCR is considered as a sensitive diagnostic tool when parasitaemia is undetectable. The slight lengthier mean of parasite and anaemia may indicate a virulent strain of T. evansi circulating throughout the country. Thus, it’s highly recommended to shed light on host-parasite relationship for better epidemiological understanding.

Download File

Full text not available from this repository.
Official URL or Download Paper: http://www.msptm.org/journal27.html

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Malaysian Society of Parasitology and Tropical Medicine
Keywords: Trypanosoma evansi; Horses; Clinical disease affecting horse
Depositing User: Azizan Arshad
Date Deposited: 20 Jan 2016 05:28
Last Modified: 20 Jan 2016 05:28
URI: http://psasir.upm.edu.my/id/eprint/29926
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item