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Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study


Murulitharan, Kavitha and Yusoff, Khatijah and Omar, Abdul Rahman and Molouki, Aidin (2013) Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study. Virus Genes, 46 (3). pp. 431-440. ISSN 0920-8569; ESSN: 1572-994X


Newcastle disease virus (NDV) strain AF2240 is a viscerotropic velogenic strain that is used as a vaccine challenge virus in Malaysia. The identification of the full length genome will be a crucial platform for further studies of this isolate. In this study, we fully sequenced the genome of a derivative of this strain named AF2240-I. The 15,192 nt long genome contains a 55-nt leader sequence at the 3′ whereas the trailer region consists of 114 nt at the 5′. The intergenic sequences between the NP-P, P-M, M-F, F-HN, and HN-L genes comprise 1, 1, 1, 31, and 47 nt, respectively. The acknowledged cleavage site of fusion protein showed amino acid sequence of 112-R-R-Q-K-R-F-117, which corresponds to those of virulent NDV strains. Phylogenetic analysis of the whole virus genome shows that the strain AF2240-I belongs to genotype VIII and is more closely related to velogenic strains QH1, QH4, Fontana, Largo, and Italienas compared to other strains of NDV. Differences are noticed in the hemagglutinin-neuraminidase (HN) and matrix (M) gene between AF2240 and its derivative AF2240-I. This is the first report of a complete genome sequence of an NDV strain isolated in Malaysia.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s11262-012-0874-y
Publisher: Springer
Keywords: Newcastle disease virus; Strain AF2240-I; Complete genome sequence
Depositing User: Azizan Arshad
Date Deposited: 20 Jan 2016 02:24
Last Modified: 20 Jan 2016 02:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11262-012-0874-y
URI: http://psasir.upm.edu.my/id/eprint/29879
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