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Mucosal genetic immunization through microsphere-based oral carriers.


Rosli, Rozita and Nograles, Nadine and Hanafi, Aimi Melati and Shamsudin, Mariana Nor and Abdullah, Syahrilnizam (2013) Mucosal genetic immunization through microsphere-based oral carriers. Human Vaccines and Immunotherapeutics, 9 (10). pp. 2222-2227. ISSN 2164-5515; ESSN: 2164-554X


Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAXI eukaryotic expression plasmid vectors carrying gene-coding sequences, either for the cholera enterotoxin B subunit (cfxB) immunostimulatory antigen or the green fluorescent protein (GFP), delivered from both types of microsphere carriers were examined in orally immunized BALB/c mice. Demonstration of transgene protein expression and IgA antibody responses at local mucosal sites suggest immunological response to a potential oral DNA vaccine formulated within the microsphere carriers.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.4161/hv.25325
Publisher: Landes Bioscience
Keywords: Alginate; Cellulose acetate phthalate; Encapsulation; IgA; Microspheres; Mucosal immunization; Plasmid DNA
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 08 Dec 2014 01:58
Last Modified: 29 Oct 2015 08:15
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4161/hv.25325
URI: http://psasir.upm.edu.my/id/eprint/29817
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