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Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers


Eshkoor, Sima Ataollahi and Ismail, Patimah and Abdul Rahman, Sabariah and Adon, Mohd Yusoff and Vasudevan, Ramachandran (2013) Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers. Toxicology Mechanisms and Methods, 23 (4). pp. 217-222. ISSN 1537-6516; ESSN: 1537-6524


Aging is attributed to both genetic and environmental factors. Occupational exposure is one of the environmental factors with potential genotoxic effects. Researchers try to determine factors involved in genetic damages at hazards exposure that could accelerate aging. Cytochrome P450 2E1 (CYP2E1) gene contributes in activation and detoxification of the environmental hazards. This polymorphism plays an important role in susceptibility of inter-individuals to DNA damage at the occupational exposure. The current study evaluated the possible influence of this gene polymorphism in aging by genomic damages through the biomarkers alterations of micronuclei (MN), comet tail length and telomere length shortening at the exposure. In this study, buccal cells were collected from the oral cavity of exposed workers and non-exposed controls. The CYP2E1 genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The wild genotype significantly affected MN frequency (p=0.007) and relative telomere length (p=0.047) in the older group of workers. It was concluded that the interaction of gene polymorphism and exposure enhances DNA damage and accelerates aging consequently.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Gerontology
DOI Number: https://doi.org/10.3109/15376516.2012.743637
Publisher: Taylor & Francis
Keywords: Aging; Comet assay; CYP2E1 polymorphism; Micronuclei; Occupational exposure
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 19 Feb 2016 02:25
Last Modified: 19 Feb 2016 02:25
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3109/15376516.2012.743637
URI: http://psasir.upm.edu.my/id/eprint/29808
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