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In vitro antibacterial and antibiofilm activities of chlorogenic acid against clinical isolates of stenotrophomonas maltophilia including the trimethoprim / sulfamethoxazole resistant strain


Citation

Karunanidhi, Arunkumar and Thomas, Renjan and Van Belkum, Alex and Neela, Vasantha Kumari (2013) In vitro antibacterial and antibiofilm activities of chlorogenic acid against clinical isolates of stenotrophomonas maltophilia including the trimethoprim / sulfamethoxazole resistant strain. BioMed Research International, 2013. art. no. 392058. pp. 1-7. ISSN 2314-6133; ESSN: 2314-6141

Abstract

The in vitro antibacterial and antibiofilm activity of chlorogenic acid against clinical isolates of Stenotrophomonas maltophilia was investigated through disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), time-kill and biofilm assays. A total of 9 clinical S. maltophilia isolates including one isolate resistant to trimethoprim / sulfamethoxazole (TMP/SMX) were tested. The inhibition zone sizes for the isolates ranged from 17 to 29 mm, while the MIC and MBC values ranged from 8 to 16 g mL-1 and 16 to 32 g mL-1. Chlorogenic acid appeared to be strongly bactericidal at 4x MIC, with a 2-log reduction in viable bacteria at 10 h. In vitro antibiofilm testing showed a 4-fold reduction in biofilm viability at 4x MIC compared to 1x MIC values (0.085 < 0.397 A 490 nm) of chlorogenic acid. The data from this study support the notion that the chlorogenic acid has promising in vitro antibacterial and antibiofilm activities against S. maltophilia.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1155/2013/392058
Publisher: Hindawi Publishing Corporation
Keywords: In vitro; Stenotrophomonas maltophilia; Chlorogenic acid; Trimethoprim/Sulfamethoxazole; Minimum inhibitory concentration(MIC); Minimum bactericidal concentration(MBC)
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 27 Dec 2014 11:05
Last Modified: 18 Sep 2015 02:53
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2013/392058
URI: http://psasir.upm.edu.my/id/eprint/29803
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