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Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection


Citation

Khrustalev, Vladislav Victorovich and Ghaznavi Rad, Ehsanollah and Neela, Vasantha Kumari and Shamsudin, Mariana Nor and Amouzandeh-Nobaveh, Alireza and Barkovsky, Eugene Victorovich (2013) Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection. Research in Microbiology, 164 (9). pp. 913-922. ISSN 0923-2508; ESSN: 1769-7123

Abstract

Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mostly due to symmetric mutational AT-pressure in the whole S. aureus genome and due to replication-associated mutational pressure characteristic of lagging strands of the "chromosome". A-T transversions would appear to be frequent in the spa gene mostly due to transcription-associated mutational pressure. Relative to other S. aureus genes, short repeats in spa are enriched by nonsense sites for G-T and A-T transversions; the probability of being nonsense for A-T transversion is high in that part of spa coding region. 13 out of 15 (87%) of the sequences with stop codons were obtained from strains isolated from patients with generalized S. aureus infection. Truncation of spa at its C-terminus is predicted to result in a protein that possesses functional IgG binding domains unable to be linked to the cell wall. This is discussed in light of the known fact that extracellular spa is a strong virulence factor involved in immune evasion.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.resmic.2013.07.003
Publisher: Elsevier
Keywords: Mutational pressure; Preterminal codons; Truncation
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 19 Feb 2016 02:24
Last Modified: 19 Feb 2016 02:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.resmic.2013.07.003
URI: http://psasir.upm.edu.my/id/eprint/29802
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