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Safety profile of dextran-spermine gene delivery vector in mouse lungs


Yeo, Wendy Wai Yeng and Hosseinkhani, Hossein and Abdul Rahman, Sabariah and Rosli, Rozita and Domb, Abraham J. and Abdullah, Syahrilnizam (2014) Safety profile of dextran-spermine gene delivery vector in mouse lungs. Journal of Nanoscience and Nanotechnology , 14 (5). pp. 3328-3336. ISSN 1533-4880;ESSN: 1533-4899


A nano-sized polymer, dextran-spermine (D-SPM), was shown to have the capacity to deliver gene to the lung of mouse via intranasal route. In this study, assessments on the safety profile of D-SPM were performed to complement the gene expression results. African green monkey kidney fibroblast (COS-7) and human adenocarcinoma breast (MCF-7) cells transfected with D-SPM/pDNA showed massive reduction in the number of viable cells. As for in vivo study, elevated level of neutrophils was observed, despite the minimal level of pro-inflammatory cytokines (TNF-α, IL-12, IFN-γ detected in the bronchoalveolar lavage fluid (BALF) of mice treated with the D-SPM/pDNA complexes. Histology profile examinations of the lungs showed mild inflammatory responses, with inflamed areas overlap with healthy areas. Although reduction of mice weight was seen at day 1 post administration, the mice did not show any sign of abnormal behavior or physical appearance. Biodistribution study was performed to determine the ability of the D-SPM/pDNA complexes to infiltrate to other non-intended organs. The result showed that the D-SPM/pDNA complexes were only localized at the lung and no gene expression was detected in other organs or blood. In short, these results indicate that the D-SPM/pDNA exhibited mild toxicity in the mouse lungs.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
Publisher: American Scientific Publishers
Keywords: Dextran-spermine; Mouse lung; Non-viral delivery; Polymer; Safety
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 27 Dec 2014 12:33
Last Modified: 22 Oct 2015 03:34
URI: http://psasir.upm.edu.my/id/eprint/29784
Statistic Details: View Download Statistic

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