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Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle


Citation

Vellasamy, Shalini and Sandrasaigaran, Pratheep and Vidyadaran, Sharmili and Abdullah, Maha and George, Elizabeth and Ramasamy, Rajesh (2013) Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle. Cell Biology International, 37 (3). pp. 250-256. ISSN 1065-6995; ESSN: 1095-8355

Abstract

Mesenchymal stem cells (MSC) generated from human umbilical cord (UC-MSC) and placenta (PLC-MSC) were assessed and compared for their immunomodulatory function on T cells proliferation by analysis of the cell cycle. Mitogen stimulated or resting T cells were co-cultured in the presence or absence of MSC. T-cell proliferation was assessed by tritiated thymidine ((3) H-TdR) assay and the mechanism of inhibition was examined bycell cycle and apoptosis assay. Both UC-MSC and PLC-MSC profoundly inhibited the proliferation of T-cell, mainly via cell-to-cell contact. MSC-mediated anti-proliferation does not lead to apoptosis,but prevented T cells from entering S phase and they therefore accumulated in the G(0) /G(1) phases. The anti-proliferative activity of MSC was related to this cell cycle arrest of T-cell. UC-MSC produced a greater inhibition than PLC-MSC in all measured parameters.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1002/cbin.10033
Publisher: International Federation for Cell Biology
Keywords: Mesenchymal stem cell; Cell cycle arrest; Umbilical cord; Placenta; T cells
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 22 May 2015 07:31
Last Modified: 03 Feb 2016 02:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/cbin.10033
URI: http://psasir.upm.edu.my/id/eprint/29676
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