Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia

Citation

Chong, Seng Shueh and Neela, Vasanthakumari and Hussin, Salasawati and Awang Hamat, Rukman (2013) Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia. Journal of Microbiological Methods, 94 (2). pp. 141-143. ISSN 0167-7012; ESSN: 1872-8359

Abstract

We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Medicine and Health Science
DOI Number:	https://doi.org/10.1016/j.mimet.2013.06.001.
Publisher:	Elsevier BV
Keywords:	Stenotrophomonas maltophilia; Pulsed Field Gel Electrophoresis; PFGE; Time-saving; Cost efficient
Depositing User:	Nurul Ainie Mokhtar
Date Deposited:	25 May 2015 05:43
Last Modified:	08 Sep 2015 04:27
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.mimet.2013.06.001.
URI:	http://psasir.upm.edu.my/id/eprint/29607
Statistic Details:	View Download Statistic

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