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Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae


Mohamed Shakrin, Nik Noorul Shakira and Balasubramaniam, Shandra Devi and Ahmad Yusof @ Hanafi, Hanani and Mastuki, Mohd Fahmi and Masri, Siti Norbaya and Mohd Taib, Niazlin and Amin Nordin, Syafinaz and Jamal, Farida Fatema @ Farida and Clarke, Stuart C. and Mohd Desa, Mohd Nasir (2013) Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae. Tropical Biomedicine, 30 (2). pp. 338-344. ISSN 0127-5720


Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsA- negative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
Publisher: Malaysian Society of Parasitology and Tropical Medicine
Keywords: Streptococcus pneumoniae; PCR; Serotypes
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 03 Aug 2016 03:36
Last Modified: 03 Aug 2016 03:36
URI: http://psasir.upm.edu.my/id/eprint/29586
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