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Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction


Citation

Yang, Chee Yong and Hamid, Sathyapriya and Wong, Mui Yun (2016) Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction. Pertanika Journal of Tropical Agricultural Science, 39 (1). pp. 55-72. ISSN 1511-3701; ESSN: 2231-8542

Abstract / Synopsis

Amplified chitinase gene sequence shared 99% homology with Musa acuminata class III acidic chitinase and beta-1,3-glucanase gene sequence was 100% homologous to Musa x paradisiaca beta-1,3-glucanase. Three nucleotide-binding sites and the leucine-rich repeat (NBS-LRR) type of putative RGCs and one serine/threonine kinase gene were characterised at the amino acid level. Kinase-2 (LVLDDVW) and kinase-3 (GSRIIITTRD) motifs in the nucleotide-binding domain were highly conserved in RGC2 and RGC3 and these genes belong to the non-TIR-NBS class RGCs. RGC1 was also clustered into non-TIR-NBS class RGCs; however, many residue substitutions were present in the kinase-2 and kinase-3 motifs. The sub-domain IX (LTEKSDVYSFGVVL) of serine/threonine protein kinase was highly conserved in RGC5 and it shared highest homology with PTH-2 from muskmelon. RT-PCR analysis revealed the differential expression of PR and RGC genes exhibited by different banana genotypes over sampling time. Chitinase was expressed during banana- FocR4 interaction in all three banana genotypes. However, its expression was high and constant in Rastali Mutiara during banana-FocR4 interaction and resulted in very low disease severity in FocR4 inoculated plants (2%) compared to Rastali wild-type (16%) and Jari Buaya (8%) at six weeks after inoculation. This suggests that chitinase may play an important role in disease resistance against FocR4. Besides, our study also shows that Rastali Mutiara can be a potential source of disease-resistant genes for molecular breeding of banana.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
Publisher: Universiti Putra Malaysia Press
Keywords: Banana; Fusarium wilt; Nucleotide-binding site; PR proteins; Resistance-gene candidate; Serine/threonine kinase
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 12 Jul 2016 11:52
Last Modified: 12 Jul 2016 11:52
URI: http://psasir.upm.edu.my/id/eprint/29584
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