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Genotyping of GATA4 gene variant (G296S) in Malaysian congenital heart disease subjects by real-time PCR high resolution melting analysis


Fawzi, Nora and Vasudevan, Ramachandran and Ismail, Patimah and Alwi, Mazeni and Abdul Aziz, Ahmad Fazli and Almeamar, Hussein and Mohamad, Nur Afiqa and Etemad, Ali (2013) Genotyping of GATA4 gene variant (G296S) in Malaysian congenital heart disease subjects by real-time PCR high resolution melting analysis. Journal of Medical Biochemistry, 32 (2). pp. 152-157. ISSN 1452-8258; ESSN: 1452-8266


Background: Congenital heart disease (CHD) is the most common birth defect; however, the underlying etiology is unrecognized in the majority of cases. GATA-binding protein 4 (GATA4), a cardiac transcription factor gene, has a crucial role in the cardiogenesis process; hence, a number of heterozygote sequence variations were identified as a cause of CHD. G296S heterozygote variant is the most frequently reported GATA4 gene sequence alteration. This study aims to investigate the role of G296S variant of the GATA4 gene in Malaysian CHD subjects. Methods: We have investigated 86 Malaysian CHD subjects with cardiac septation defects for the presence of the GATA4 gene heterozygote variant (G296S) by the new technology of high resolution melting (HRM) analysis. Results: Genotyping of G296S (c.886G>A) by HRM analysis shows that all the sample genotypes were of the wild GG type genotype and the heterozygote mutant GA genotype was totally absent from this study cohort. Conclusions: The results of our study showed that the G296S variant of the GATA4 gene was not associated with the development of CHD in Malaysian subjects. The use of HRM analysis proved a cost-effective, high-throughput, specific and sensitive genotyping technique which eliminates the need for unnecessary sequencing.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.2478/jomb-2013-0006
Publisher: De Gruyter Open
Keywords: Congenital heart disease; Heterozygote; Variant; Cardiac transcription factor; Real-time high resolution melting analysis
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 08 Dec 2015 07:04
Last Modified: 08 Dec 2015 07:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2478/jomb-2013-0006
URI: http://psasir.upm.edu.my/id/eprint/29558
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