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Characterization of cellulolytic bacterial cultures grown in different substrates


Citation

Alshelmani, Mohamed Idris and Loh, Teck Chwen and Foo, Hooi Ling and Lau, Wei Hong and Sazili, Awis Qurni (2013) Characterization of cellulolytic bacterial cultures grown in different substrates. The Scientific World Journal, 2013. art. no. 689235. pp. 1-6. ISSN 2356-6140; ESSN: 1537-744X

Abstract / Synopsis

Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (SSF) of palm kernel cake (PKC). The bacteria cultures were grown on reconstituted nutrient broth, incubated at 30∘C and agitated at 200 rpm. Carboxymethyl cellulase, xylanase, and mannanase activities were determined using different substrates and after SSF of PKC. The SSF was conducted for 4 and 7 days with inoculum size of 10% (v/w) on different PKC concentration-to-moisture ratios: 1 : 0.2, 1 : 0.3, 1 : 0.4, and 1 : 0.5. Results showed that Bacillus amyloliquefaciens 1067 DSMZ, Bacillus megaterium 9885 ATCC, Paenibacillus curdlanolyticus 10248 DSMZ, and Paenibacillus polymyxa 842 ATCC produced higher enzyme activities as compared to other bacterial cultures grown on different substrates. The cultures mentioned above also produced higher enzyme activities when they were incubated under SSF using PKC as a substrate in different PKC-to-moisture ratios after 4 days of incubation, indicating that these cellulolytic bacteria can be used to degrade and improve the nutrient quality of PKC.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.1155/2013/689235
Publisher: Hindawi Publishing Corporation
Keywords: Bacteria; Cellulolytic bacterial cultures; Cellulose; Substrate; Solid state fermentation; Palm kernel cake.
Depositing User: Norhazura Hamzah
Date Deposited: 14 Aug 2014 02:31
Last Modified: 12 Feb 2016 03:33
URI: http://psasir.upm.edu.my/id/eprint/29494
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