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Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians


Etemad, Ali and Ramachandran, Vasudevan and Pishva, Seyyed Reza and Heidari, Farzad and Abdul Aziz, Ahmad Fazli and Mohamed Yusof, Ahmad Khairuddin and Chong, Pei Pei and Ismail, Patimah (2013) Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians. International Journal of Molecular Sciences, 14 (9). pp. 19230-19244. ISSN 1422-0067


Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Mellitus (T2DM), as well as in cardiovascular diseases (CVD). The Leptin Receptor (LEPR) gene and its polymorphisms have not been extensively studied in relation to the T2DM and its complications in various populations. In this study, we have determined the association of Gln223Agr loci of LEPR gene in three ethnic groups of Malaysia, namely: Malays, Chinese and Indians. A total of 284 T2DM subjects and 281 healthy individuals were recruited based on International Diabetes Federation (IDF) criteria. Genomic DNA was extracted from the buccal specimens of the subjects. The commercial polymerase chain reaction (PCR) method was carried out by proper restriction enzyme MSP I to both amplify and digest the Gln223Agr polymorphism. The p-value among the three studied races was 0.057, 0.011 and 0.095, respectively. The values such as age, WHR, FPG, HbA1C, LDL, HDL, Chol and Family History were significantly different among the subjects with Gln223Agr polymorphism of LEPR (p < 0.05).

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Official URL or Download Paper: http://www.mdpi.com/1422-0067/14/9/19230

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Gerontology
DOI Number: https://doi.org/10.3390/ijms140919230
Publisher: MDPI
Keywords: Leptin receptor; Polymorphism; Type 2 diabetes mellitus; Malaysia; Restriction enzyme
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 12 Jul 2016 09:39
Last Modified: 12 Jul 2016 09:39
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/ijms140919230
URI: http://psasir.upm.edu.my/id/eprint/29466
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