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Identification, characterisation and phylogenetic analysis of commensal bacteria isolated from human breast milk in Malaysia


Hassan, Zubaida and Mustafa, Shuhaimi and Abdul Rahim, Raha and Mat Isa, Nurulfiza (2016) Identification, characterisation and phylogenetic analysis of commensal bacteria isolated from human breast milk in Malaysia. Pertanika Journal of Science & Technology, 24 (2). pp. 351-370. ISSN 0128-7680; ESSN: 2231-8526


Human breast milk microbiota is essential for infant immune system development, maturation and protection against infection. However, there is scarce information on the fluid’s microbiological composition from Malaysia. The objective of the study was to isolate, identify and characterise commensal bacterial population present in human breast milk from Malaysia. One hundred bacteria were isolated from the human breast milk of healthy lactating women (n=30). After preliminary screening, 20 isolates were characterised using both phenotypic and molecular techniques. The results indicated that most frequently identified bacteria in this study were E. faecalis and S. hominis. These organisms alongside E. cloacae were all metabolised D-Maltose, Sucrose, D-Turanose, α-D-Glucose, D-Fructose, D-Mannose, D-Galactose, D-sorbitol and D-Mannitol and were able to grow at pH 5 and 6, 1% sodium lactate, 1%, 2% and 8% NaCl. BLAST showed over 99% similarity to those deposited in Genbank. Phylogenetic-relatedness was depicted using neighbour-joining method and had two clades with 100% bootstrap. These findings provided insight into the nature, characteristics and also phylogenetic-relatedness of bacteria present in human milk from Malaysia. Isolation and identification of commensal bacteria from human milk are considered the first step for future studies on the benefit of these organisms towards human health.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Halal Products Research Institute
Publisher: Universiti Putra Malaysia Press
Keywords: Commensal bacteria; Human breast milk; Malaysia; Phenotype; Phylogeny
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 02 Aug 2016 03:05
Last Modified: 02 Aug 2016 03:05
URI: http://psasir.upm.edu.my/id/eprint/29424
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