Citation
Abstract
The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. Seventeen Lactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using selective media. The 16S rRNA genes from the extracted DNA of bacterial colonies were amplified with PCR using universal bacteria primers. All bacterial 16S rRNA genes were sequenced, aligned and the distant bacteria were deposited in GenBank. The lactobacilli strains were identified as Lactobacillus spp. related-sequences (64.15%) with other abundant sequences being related to Lactobacillus kunkeei (34.85%). The findings revealed that Apis dorsata honey comb has potential to be a source of new bacteria with probiotic activities in honey bee or as natural food preservatives for the food industry.
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Official URL or Download Paper: http://www.tandfonline.com/doi/abs/10.3896/IBRA.1....
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Faculty of Food Science and Technology |
| DOI Number: | https://doi.org/10.3896/IBRA.1.52.5.10 |
| Publisher: | International Bee Research Association |
| Keywords: | 16S rRNA gene; Apis dorsata; Honey comb; Identification; Lactobacillus; Probiotics; Sequencing |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 13 Jul 2016 02:26 |
| Last Modified: | 13 Jul 2016 02:26 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3896/IBRA.1.52.5.10 |
| URI: | http://psasir.upm.edu.my/id/eprint/29319 |
| Statistic Details: | View Download Statistic |
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