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Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment


Syahputra, Bambang Surya Adji and Sinniah, Uma Rani and Syed Rastan, Syed Omar and Ismail, Mohd Razi (2013) Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment. Journal of Food and Pharmaceutical Sciences, 1 (1). pp. 14-17. ISSN 2089-7200; ESSN: 2339-0948


The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy.

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Official URL or Download Paper: http://journal.ugm.ac.id/jfps/article/view/1841

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
Publisher: Gadjah Mada University
Keywords: Analysis; Gibberellic acid; Paclobutrazol; HPLC
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 26 Apr 2016 01:38
Last Modified: 26 Apr 2016 01:38
URI: http://psasir.upm.edu.my/id/eprint/29240
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