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Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species


Far, Hadi Zokaei and Saad, Che Roos and Mohd Daud, Hassan and Kamarudin, Mohd Salleh and Fard, Ehsan Ramezani (2013) Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species. Asian Journal of Animal and Veterinary Advances, 8 (2). pp. 293-300. ISSN 1683-9919; ESSN: 1996-3289


The emergence of antibiotic-resistant bacterial pathogen has led aquaculture attentions to the use of probiotics as an alternative to antibiotics. This study was conducted to isolate bacterial micro flora from digestive tract of healthy juvenile white shrimp, Litopenaeus vannamei based on antagonistic activity against shrimp pathogen, Vibrio parahaemolyticus. In this study, potential probiotic strains were isolated using replica plating method to screen the bacteria with antagonism properties. Six isolates were identified as Pseudomonas sp. using conventional biochemical tests and Biolog GN microplates. In addition three Vibrio species including V. parahaemolyticus, Vibrio alginolyticus and Vibrio cholerae were isolated from moribund shrimp and used as pathogen in this study. The pathogenicity of three Vibrio species was tested on shrimp L. vannamei by injecting 0.1 mL of 107 CFU mL-1 of each pathogen into the third abdominal segment. Among three Vibrio species only V. parahaemolyticus found to be highly virulent to shrimp with 43% cumulated mortality after 10 days. Subsequently, all Pseudomonas sp. isolates were tested for antibacterial activity against three Vibrio species using cross streak assay. Strong antibacterial activity was recorded for Pseudomonas sp. isolates number 5, 7, 15 and 30 against three pathogens. In addition a reasonable antibacterial activity was observed for isolates number 9 and 12. On the basis of great antibacterial activity of Pseudomonas sp. isolates these species may be considered for future challenge experiments in shrimp as a very promising alternative to the use of antibiotics.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.3923/ajava.2013.293.300
Publisher: Academic Journals
Keywords: Antibacterial activity; Litopenaeus uannamei; Pseudomonas sp.; Vibriosis
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 20 Jun 2016 07:05
Last Modified: 20 Jun 2016 07:05
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajava.2013.293.300
URI: http://psasir.upm.edu.my/id/eprint/29213
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