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Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology


Tomizawa, Yuki and Shinmura, Yoshimi and Wee, Alison K. S. and Takayama, Koji and Asakawa, Takeshi and Yllano, Orlex Baylen and Salmo III, Severino Garengo and Ardli, Erwin Riyanto and Tung, Nguyen Xuan and Malekal, Norhaslinda and Onrizal, and Meenakshisundaram, Sankararamasubramanian Halasya and Sungkaew, Sarawood and Saleh @ Japri, Mohd Nazre and Adjie, Bayu and Soe, Khin Khin and Oguri, Emiko and Murakami, Noriaki and Watano, Yasuyuki and Baba, Shigeyuki and Webb, Edward L. and Kajita, Tadashi (2013) Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology. Conservation Genetics Resources, 5 (4). pp. 1159-1162. ISSN 1877-7252; ESSN: 1877-7260


Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Forestry
DOI Number: https://doi.org/10.1007/s12686-013-9990-9
Publisher: Springer
Keywords: Conservation; Genetic diversity; Indo-West Pacific; Mangrove; Pyrosequencing; SSR
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 25 Apr 2016 02:46
Last Modified: 25 Apr 2016 02:46
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12686-013-9990-9
URI: http://psasir.upm.edu.my/id/eprint/29127
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