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Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis.


Citation

Baradaran, Ali and Sieo, Chin Chin and Foo, Hooi Ling and Md. Illias, Rosli and Yusoff, Khatijah and Abdul Rahim, Raha (2013) Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. Biotechnology Letters, 35 (2). pp. 233-238. ISSN 0141-5492; ESSN: 1573-6776

Abstract / Synopsis

Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (GFP) and cloned into Lactococcus lactis pNZ8048 and pMG36e vectors, respectively. Western blot analysis indicated that the GFP proteins were secreted using both heterologous SPs. ELISA showed that the secretion efficiency of GFP using Spk1 (0.64 μg/ml) was similar to using Usp45 (0.62 μg/ml) and Spk3 (0.58 μg/ml).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s10529-012-1059-4
Publisher: Springer Netherlands
Keywords: Green fluorescent protein; Lactococcus lactis; Secretion system; Signal peptides.
Depositing User: Umikalthom Abdullah
Date Deposited: 28 May 2014 09:00
Last Modified: 24 Aug 2015 10:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10529-012-1059-4
URI: http://psasir.upm.edu.my/id/eprint/28111
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