Citation
Lin, Yu Kiat and Ooi, Chien Wei and Tan, Joo Shun and Pau, Loke Show and Ariff, Arbakariya and Ling, Tau Chuan
(2013)
Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems.
Separation and Purification Technology, 120.
pp. 362-366.
ISSN 1383-5866; ESSN: 1873-3794
Abstract
The purification of intracellular human recombinant interferon-alpha2b (IFN-α2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-based bottom phase (ammonium sulfate, di-potassium hydrogen phosphate and monosodium citrate) on IFN-α2b purification were studied. The results showed that the optimum condition for purification of IFN-α2b was achieved in ATPS composed of 18% (w/w) 2-propanol with 22% (w/w) ammonium sulfate in the presence of 1% (w/w) sodium chloride (NaCl). The purified IFN-α2b recorded a purification factor (PF) of 16.24 with the yield of 74.64%.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Institute of Bioscience |
| DOI Number: | https://doi.org/10.1016/j.seppur.2013.09.038 |
| Publisher: | Elsevier |
| Keywords: | Aqueous two-phase system; Interferon-alpha2b; Alcohol/salt system; Purification |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 20 Jun 2016 05:55 |
| Last Modified: | 20 Jun 2016 05:55 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.seppur.2013.09.038 |
| URI: | http://psasir.upm.edu.my/id/eprint/28099 |
| Statistic Details: | View Download Statistic |
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