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Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system


Citation

Ng, Hui Suan and Ooi, Chien Wei and Mokhtar, Mohd Noriznan and Show, Pau Loke and Ariff, Arbakariya and Tan, Joo Shun and Ng, Eng Poh and Ling, Tau Chuan (2013) Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system. Bioresource Technology, 142. pp. 723-726. ISSN 0960-8524; ESSN: 1873-2976

Abstract

An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8 h. An average total CDs concentration of 13.7 mg/mL, (4.77 mg/mLα-CD, 5.02 mg/mLβ-CD and 3.91 mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.biortech.2013.05.087
Publisher: Elsevier
Keywords: Aqueous two-phase system; Extractive bioconversion; Cyclodextrin; Bacillus cereus; Cyclodextrin glycosyltransferase
Depositing User: Nabilah Mustapa
Date Deposited: 21 Apr 2016 09:37
Last Modified: 21 Apr 2016 09:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.biortech.2013.05.087
URI: http://psasir.upm.edu.my/id/eprint/28078
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