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Segregation and genetic linkage analyses of river catfish, Mystus nemurus, based on microsatellite markers


Citation

Hoh, Boon Peng and Siraj, Siti Shapor and Tan, Soon Guan and Yusoff, Khatijah (2013) Segregation and genetic linkage analyses of river catfish, Mystus nemurus, based on microsatellite markers. Genetics and Molecular Research, 12 (3). pp. 2578-2593. ISSN 1676-5680

Abstract

The river catfish Mystus nemurus is an important fresh water species for aquaculture in Malaysia. We report the first genetic linkage map of M. nemurus based on segregation analysis and a linkage map using newly developed microsatellite markers of M. nemurus. A total of 70 of the newly developed polymorphic DNA microsatellite markers were analyzed on pedigrees generated using a pseudo-testcross strategy from 2 mapping families. In the first mapping family, 100 offspring were produced from randomly selected dams of the same populations; dams of the second family were selected from 2 different populations, and this family had 50 offspring. Thirty-one of the 70 markers segregated according to the Mendelian segregation ratio. Linkage analysis revealed that 17 microsatellite markers belonging to 7 linkage groups were obtained at a logarithm of the odds score of 1.2 spanning 584 cM by the Kosambi mapping function, whereas the other 14 remained unlinked. The results from this study will act as primer to a more extensive genetic mapping study aimed towards identifying genetic loci involved in determining economically important traits.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Agriculture
Institute of Bioscience
DOI Number: https://doi.org/10.4238/2013.February.28.1
Publisher: Fundacao de Pesquisas Cientificas de Ribeirao Preto
Keywords: Linkage analysis; Microsatellites; Mystus nemurus; Pseudo-testcross strategy; River catfish; Segregation
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 20 Jun 2016 03:08
Last Modified: 20 Jun 2016 03:08
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4238/2013.February.28.1
URI: http://psasir.upm.edu.my/id/eprint/28059
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