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Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility


Citation

Thuc, Le Vinh and Geelen, Danny and Ky, Huynh and Ooi, Siew Eng and Napis, Suhaimi and Sinniah, Uma Rani and Namasivayam, Parameswari (2013) Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility. Molecular Biology Reports, 40 (2). pp. 1579-1590. ISSN 0301-4851; ESSN: 1573-4978

Abstract

The functional analysis of the TAPETUM DEVELOPMENT1-like analog Eg707 of oil palm was carried out in rice by over-expressing Eg707 under the control of a double cauliflower mosaic virus 35S promoter. Ectopic expression of Eg707 in rice induced dark green and matured compact brownish calli compared to pale wild type and negative control calli. Regenerated transgenic rice plants exhibited a reduction in organ size and plant height, rolled, erect leaves, less tillers, increased chlorophyll content, and reduced fertility with smaller green seeds. At the molecular level Eg707 overexpression caused an increase in the transcription of SAPK9, a SnRK2 protein kinase family member that is activated by ABA and hyperosmotic stress. Together, the results show that ectopic Eg707 expression influences cell division and differentiation, presumably via altered hormone homeostasis.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.1007/s11033-012-2206-7
Publisher: Springer
Keywords: Oil palm; Abscisic acid; Hormone signaling; Tissue culture
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 07 Mar 2016 05:12
Last Modified: 07 Mar 2016 05:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11033-012-2206-7
URI: http://psasir.upm.edu.my/id/eprint/28009
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