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A method for purification of intracellular protein.


Tey Beng Ti (2009) A method for purification of intracellular protein. UNSPECIFIED.

Abstract / Synopsis

The present specification discloses an integrated purification technique for purifying recombinant intracellular protein (GFP) from intact E. coli cells using preparative PAGE column. Under the influence of electric fields, the negatively charged GFP was drifted out from cells without the need of cell disruption process. The electrophoresed cells were observed under SEM and some on the membrane of cells were observed. GFP was then separated from other host cells host protein along polyacrylamide gel and finally eluted electrophoretically from the gel. The invention has successfully recovered the GFP with purity and yeild 95% and 82%, respectively in 100 min operation.

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Additional Metadata

Item Type: Patent
Divisions: Faculty of Engineering
Keywords: Purifying recombinant intracellular protein; Purification technique; Cell.
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 02 Apr 2014 10:37
Last Modified: 02 Apr 2014 10:37
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