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Quality of salvaged epididymal spermatozoa in local dogs


Jacob, Telma Dora and Dhaliwal, Gurmeet Kaur and Yusoff, Rosnina and Lim, Suit Fun (2010) Quality of salvaged epididymal spermatozoa in local dogs. In: 5th Seminar on Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. .


The quality and quantity of epididymal spermatozoa and their relationship to scrotal circumference and testicular weight for local dogs in Malaysia were successfully determined. The longevity of spermatozoa in an alternative storage medium was also determined. The salvage of epididymal spermatozoa was done in twenty-two humanely euthanised adult local dogs. The overall mean ± SEM of the total number, general motility, progressive motility, and percentage live and abnormal spermatozoa of cauda epididymal spermatozoa salvaged per dog were 408 ± 55.31 x 106, 80.91 ± 1.84%, 46.25 ± 3.34%, 72.43 ± 1.47% and 6.80 ± 0.95%, respectively. The scrotal circumference and testicular weight were not appropriate indicators of the number of salvageable cauda epididymal spermatozoa as indicated by moderate correlation coefficients of 0.545 and 0.546, respectively. There was a significant difference (P<0.05) in general motility, progressive motility and live percentage of spermatozoa during storage (4-8ºC) between an extender based on 0.9% NaCl and a commercial canine semen extender over time. However, for the first 2 days, these parameters were similar in value. Therefore, 0.9% NaCl should be further investigated as a potential medium for short term storage of epididymal spermatozoa.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Veterinary Medicine
Keywords: Canine; Epididymal spermatozoa; Scrotal circumference; Testicular weight; Extender
Depositing User: Azizan Arshad
Date Deposited: 05 May 2014 01:53
Last Modified: 08 Jan 2015 10:23
URI: http://psasir.upm.edu.my/id/eprint/27657
Statistic Details: View Download Statistic

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